The effect of high-energy electron irradiation on the temperature dependences of the resistivity ρ(T), fluctuation conductivity (FLC), and pseudogap (PG) Δ*(T) of (YBCO) single crystals containing virtually no twins was studied. A linear increase in the resistivity and a linear decrease in the superconducting (SC) transition temperature with increasing irradiation doses φ were observed. For relatively small φ, the linear can be described by the Abrikosov-Gorkov (AG) pair breaking theory, and for large φ, by the Emery-Kivelson (EK) theory, which takes into account the suppression of by quantum phase fluctuations caused by irradiation defects. As FLC shows, at the average value of , which corresponds to the AG-EK crossover, the distance between the conducting planes, , as well as the coherence lengths along the axis, (0), and the region of SC fluctuations, , increase sharply, and the two-dimensional contribution of the Maki-Thompson fluctuations (2D-MT) unexpectedly changes to the two-dimensional contribution of the Aslamazov-Larkin (2D-AL). Surprisingly, no features in ρ(φ) and (φ) indicating the AG-EK crossover are observed. At the same time, at , a sharp increase in the opening temperature of PG, T*, as well as the value of PG, Δ*, is observed, which indicates a possible decrease in DOS under the influence of defects. With a further increase in φ, all the parameters of PG and its dimensions are greatly reduced, and an unusual shape of Δ*(T) is found. However, quite unexpectedly, at the temperature dependences of both FLC and PG demonstrate curves typical for well-structured YBCO, regardless of the number of defects.