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X-ray phase contrast imaging with kilometer propagation distance within a meter

Rebecca Spiecker*

Martin Spiecker

Adyasha Biswal

Mykola Shcherbinin

Tilo Baumbach

  • *Contact author: rebecca.spiecker@kit.edu
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Phys. Rev. Research 7, 043315 – Published 19 December, 2025

DOI: https://doi.org/10.1103/pkmv-x47f

Abstract

We introduce an x-ray imaging technique to facilitate dose-efficient propagation-based phase contrast imaging (PB-PCI) of large, centimeter-sized samples. The dose efficiency is realized by generating image contrast for low sampling frequencies, i.e., large pixel sizes, which would require propagation distances of tens to hundreds of meters in conventional PB-PCI. By magnifying the spatial frequency distribution of the diffracted wave field behind the sample, we strongly enhance the image contrast at short physical propagation distances, corresponding to kilometer-long distances. For this purpose, the wave field is demagnified by asymmetric Bragg crystal optics. Concomitantly, the crystal arrangement substantially reduces image blur caused by the finite x-ray source size. We demonstrate experimentally the significant increase in image contrast compared to conventional PB-PCI at the same short physical propagation distance. In combination with a subsequent Bragg magnifier, the method will allow for an even higher dose efficiency, rendering this technique a potential candidate for, e.g., low-dose (bio)medical diagnostics.

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References (84)

  1. T. Salditt, T. Aspelmeier, and S. Aeffner, Biomedical Imaging (De Gruyter, Berlin, Germany, 2017), p. 244
  2. M. Endrizzi, X-ray phase-contrast imaging, Nucl. Instrum. Methods Phys. Res., Sect. A 878, 88 (2018).
  3. H. N. Chapman and K. A. Nugent, Coherent lensless x-ray imaging, Nat. Photonics 4, 833 (2010).
  4. F. Pfeiffer, J. Herzen, M. Willner, M. Chabior, S. Auweter, M. Reiser, and F. Bamberg, Grating-based x-ray phase contrast for biomedical imaging applications, Z. Med. Phys. 23, 176 (2013).
  5. V. V. Lider and M. V. Kovalchuk, X-ray phase-contrast methods, Crystallogr. Rep. 58, 769 (2013).
  6. S. Lang, I. Zanette, M. Dominietto, M. Langer, A. Rack, G. Schulz, G. Le Duc, C. David, J. Mohr, F. Pfeiffer, B. Müller, and T. Weitkamp, Experimental comparison of grating- and propagation-based hard x-ray phase tomography of soft tissue, J. Appl. Phys. 116, 154903 (2014).
  7. S. Tao, C. He, X. Hao, C. Kuang, and X. Liu, Principles of different x-ray phase-contrast imaging: A review, Appl. Sci. 11, 2971 (2021).
  8. S. C. Mayo, A. W. Stevenson, and S. W. Wilkins, In-line phase-contrast x-ray imaging and tomography for materials science, Materials 5, 937 (2012).
  9. M. Cotte, P.-O. Autran, C. Berruyer, C. Dejoie, J. Susini, and P. Tafforeau, Cultural and natural heritage at the ESRF: Looking back and to the future, Synchrotron Radiat. News 32, 34 (2019).
  10. O. Betz, U. Wegst, D. Weide, M. Heethoff, L. Helfen, W.-K. Lee, and P. Cloetens, Imaging applications of synchrotron x-ray phase-contrast microtomography in biological morphology and biomaterials science. I. General aspects of the technique and its advantages in the analysis of millimetre-sized arthropod structure, J. Microsc. 227, 51 (2007).
  11. J. Moosmann, A. Ershov, V. Altapova, T. Baumbach, M. S. Prasad, C. LaBonne, X. Xiao, J. Kashef, and R. Hofmann, X-ray phase-contrast in vivo microtomography probes new aspects of Xenopus gastrulation, Nature (London) 497, 374 (2013).
  12. R. A. Lewis, Medical phase contrast x-ray imaging: Current status and future prospects, Phys. Med. Biol. 49, 3573 (2004).
  13. A. Bravin, P. Coan, and P. Suortti, X-ray phase-contrast imaging: From pre-clinical applications towards clinics, Phys. Med. Biol. 58, R1 (2013).
  14. J. Kastner, C. Heinzl, B. Plank, D. Salaberger, C. Gusenbauer, and S. Senck, New x-ray computed tomography methods for research and industry, in 7th Conference on Industrial Computed Tomography (iCT2017) (2017), Vol. 22.
  15. S. T. Taba, T. E. Gureyev, M. Alakhras, S. Lewis, D. Lockie, and P. C. Brennan, X-ray phase-contrast technology in breast imaging: Principles, options, and clinical application, Am. J. Roentgenol. 211, 133 (2018).
  16. M. Eckermann, J. Frohn, M. Reichardt, M. Osterhoff, M. Sprung, F. Westermeier, A. Tzankov, C. Werlein, M. Kühnel, D. Jonigk, and T. Salditt, 3D virtual pathohistology of lung tissue from COVID-19 patients based on phase contrast x-ray tomography, eLife 9, e60408 (2020).
  17. T. J. Davis, D. Gao, T. Gureyev, A. Stevenson, and S. Wilkins, Phase-contrast imaging of weakly absorbing materials using hard x-rays, Nature (London) 373, 595 (1995).
  18. D. Chapman, W. Thomlinson, R. Johnston, D. Washburn, E. Pisano, N. Gmür, Z. Zhong, R. Menk, F. Arfelli, and D. Sayers, Diffraction enhanced x-ray imaging, Phys. Med. Biol. 42, 2015 (1997).
  19. C. David, B. Nöhammer, H. H. Solak, and E. Ziegler, Differential x-ray phase contrast imaging using a shearing interferometer, Appl. Phys. Lett. 81, 3287 (2002).
  20. A. Momose, S. Kawamoto, I. Koyama, Y. Hamaishi, K. Takai, and Y. Suzuki, Demonstration of x-ray Talbot interferometry, Jpn. J. Appl. Phys. 42, L866 (2003).
  21. K. S. Morgan, D. M. Paganin, and K. K. W. Siu, X-ray phase imaging with a paper analyzer, Appl. Phys. Lett. 100, 124102 (2012).
  22. M.-C. Zdora, State of the art of x-ray speckle-based phase-contrast and dark-field imaging, J. Imaging 4, 60 (2018).
  23. A. Snigirev, I. Snigireva, V. Kohn, S. Kuznetsov, and I. Schelokov, On the possibilities of x-ray phase contrast microimaging by coherent high-energy synchrotron radiation, Rev. Sci. Instrum. 66, 5486 (1995).
  24. S. W. Wilkins, T. E. Gureyev, D. Gao, A. Pogany, and A. W. Stevenson, Phase-contrast imaging using polychromatic hard x-rays, Nature (London) 384, 335 (1996).
  25. P. Cloetens, W. Ludwig, J. Baruchel, D. Van Dyck, J. Van Landuyt, J. Guigay, and M. Schlenker, Holotomography: Quantitative phase tomography with micrometer resolution using hard synchrotron radiation X rays, Appl. Phys. Lett. 75, 2912 (1999).
  26. A. Zamir, O. J. Arthurs, C. K. Hagen, P. C. Diemoz, T. Brochard, A. Bravin, N. J. Sebire, and A. Olivo, X-ray phase contrast tomography; proof of principle for post-mortem imaging, Br. J. Radiol. 89, 20150565 (2016).
  27. W. L. Wagner, F. Wuennemann, S. Pacilé, J. Albers, F. Arfelli, D. Dreossi, J. Biederer, P. Konietzke, W. Stiller, M. O. Wielpütz et al., Towards synchrotron phase-contrast lung imaging in patients—A proof-of-concept study on porcine lungs in a human-scale chest phantom, J. Synchrotron Radiat. 25, 1827 (2018).
  28. M. Donnelley, K. S. Morgan, R. Gradl, M. Klein, D. Hausermann, C. Hall, A. Maksimenko, and D. W. Parsons, Live-pig-airway surface imaging and whole-pig CT at the Australian Synchrotron Imaging and Medical Beamline, J. Synchrotron Radiat. 26, 175 (2019).
  29. G. E. Barbone, A. Bravin, A. Mittone, S. Grosu, J. Ricke, G. Cavaletti, V. Djonov, and P. Coan, High-spatial-resolution three-dimensional imaging of human spinal cord and column anatomy with postmortem x-ray phase-contrast micro-CT, Radiology (2020).
  30. C. Walsh, P. Tafforeau, W. Wagner, D. Jafree, A. Bellier, C. Werlein, M. Kühnel, E. Boller, S. Walker-Samuel, J. Robertus et al., Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography, Nat. Methods 18, 1532 (2021).
  31. P. Baran, S. Pacile, Y. I. Nesterets, S. C. Mayo, C. Dullin, D. Dreossi, F. Arfelli, D. Thompson, D. Lockie, M. McCormack et al., Optimization of propagation-based x-ray phase-contrast tomography for breast cancer imaging, Phys. Med. Biol. 62, 2315 (2017).
  32. L. Broche, P. Pisa, L. Porra, L. Degrugilliers, A. Bravin, M. Pellegrini, J. B. Borges, G. Perchiazzi, A. Larsson, G. Hedenstierna, and S. Bayat, Individual airway closure characterized in vivo by phase-contrast CT imaging in injured rabbit lung, Crit. Care Med. 47, e774 (2019).
  33. C. Tavakoli, E. Cuccione, C. Dumot, D. Cormode, M. Wiart, H. Elleaume, and E. Brun, Tracking cells in the brain of small animals using synchrotron multi-spectral phase contrast imaging, in Medical Imaging 2021: Physics of Medical Imaging (SPIE, Bellingham, Washington, USA, 2021), Vol. 11595, pp. 1218–1224.
  34. B. D. Arhatari, A. W. Stevenson, B. Abbey, Y. I. Nesterets, A. Maksimenko, C. J. Hall, D. Thompson, S. C. Mayo, T. Fiala, H. M. Quiney et al., X-ray phase-contrast computed tomography for soft tissue imaging at the Imaging and Medical Beamline (IMBL) of the Australian Synchrotron, Appl. Sci. 11, 4120 (2021).
  35. M. Cotte, K. Dollman, V. Fernandez, V. Gonzalez, F. Vanmeert, L. Monico, C. Dejoie, M. Burghammer, L. Huder, S. Fisher et al., New opportunities offered by the ESRF to the cultural and natural heritage communities, Synchrotron Radiat. News 35, 3 (2022).
  36. I. Häggmark, K. Shaker, S. Nyrén, B. Al-Amiry, E. Abadi, W. P. Segars, E. Samei, and H. M. Hertz, Phase-contrast virtual chest radiography, Proc. Natl. Acad. Sci. USA 120, e2210214120 (2023).
  37. J. Albers, W. L. Wagner, M. O. Fiedler, A. Rothermel, F. Wünnemann, F. Di Lillo, D. Dreossi, N. Sodini, E. Baratella, M. Confalonieri, F. Arfelli, A. Kalenka, J. Lotz, J. Biederer, M. O. Wielpütz, H.-U. Kauczor, F. Alves, G. Tromba, and C. Dullin, High resolution propagation-based lung imaging at clinically relevant x-ray dose levels, Sci. Rep. 13, 4788 (2023).
  38. I. Gunaseelan, A. Amin Zadeh, B. Arhatari, A. Maksimenko, C. Hall, D. Hausermann, B. Kumar, J. Fox, H. Quiney, D. Lockie, S. Lewis, P. Brennan, T. Gureyev, and S. Tavakoli Taba, Propagation-based phase-contrast imaging of the breast: Image quality and the effect of x-ray energy and radiation dose, Br. J. Radiol. 96, 20221189 (2023).
  39. F. Cianciosi, A.-L. Buisson, P. Tafforeau, and P. Van Vaerenbergh, BM18, the new ESRF-EBS beamline for hierarchical phase-contrast tomography, in Proc. MEDSI'20, Chicago, IL, USA (JACOW Publishing, Geneva, Switzerland, 2021), pp. 1–5.
  40. T. Lang, N. Saeidnezhad, K. Dremel, D. Weller, M. Diez, A. M. Stock, T. Sauer, F. Cianciosi, C. Jarnias, P. Tafforeau, and S. Zabler, Multiscale phase-contrast tomography at BM18, e-J. Nondestr. Test. 28 (2023).
  41. J. M. Cowley, Electron microdiffraction, in Advances in Electronics and Electron Physics, edited by L. Marton (Academic Press, Cambridge, MA, 1978), Vol. 46, pp. 1–53.
  42. R. Spiecker, P. Pfeiffer, A. Biswal, M. Shcherbinin, M. Spiecker, H. Hessdorfer, M. Hurst, Y. Zharov, V. Bellucci, T. Farago et al., Dose-efficient in vivo x-ray phase contrast imaging at micrometer resolution, Optica 10, 1633 (2023).
  43. S. Si-Mohamed, S. Boccalini, P.-A. Rodesch, R. Dessouky, E. Lahoud, T. Broussaud, M. Sigovan, D. Gamondes, P. Coulon, Y. Yagil, L. Boussel, and P. Douek, Feasibility of lung imaging with a large field-of-view spectral photon-counting CT system, Diagn. Interventional Imaging 102, 305 (2021).
  44. W. J. Boettinger, H. E. Burdette, and M. Kuriyama, X-ray magnifier, Rev. Sci. Instrum. 50, 26 (1979).
  45. K. Kobayashi, K. Izumi, H. Kimura, S. Kimura, T. Ibuki, Y. Yokoyama, Y. Tsusaka, Y. Kagoshima, and J. Matsui, X-ray phase-contrast imaging with submicron resolution by using extremely asymmetric Bragg diffractions, Appl. Phys. Lett. 78, 132 (2001).
  46. R. D. Spal, Submicrometer resolution hard x-ray holography with the asymmetric Bragg diffraction microscope, Phys. Rev. Lett. 86, 3044 (2001).
  47. M. Stampanoni, G. Borchert, R. Abela, and P. Rüegsegger, Bragg magnifier: A detector for submicrometer x-ray computer tomography, J. Appl. Phys. 92, 7630 (2002).
  48. P. Modregger, D. Lübbert, P. Schäfer, and R. Köhler, Magnified x-ray phase imaging using asymmetric Bragg reflection: Experiment and theory, Phys. Rev. B 74, 054107 (2006).
  49. P. Vagovič, D. Korytár, P. Mikulík, A. Cecilia, C. Ferrari, Y. Yang, D. Hänschke, E. Hamann, D. Pelliccia, T. A. Lafford, M. Fiederle, and T. Baumbach, In-line Bragg magnifier based on V-shaped germanium crystals, J. Synchrotron Radiat. 18, 753 (2011).
  50. K. Hirano, Y. Takahashi, and H. Sugiyama, Development and application of variable-magnification x-ray Bragg magnifiers, Nucl. Instrum. Methods Phys. Res., Sect. A 741, 78 (2014).
  51. N. Watanabe, M. Suzuki, Y. Higashi, and N. Sakabe, Rotated-inclined focusing monochromator with simultaneous tuning of asymmetry factor and radius of curvature over a wide wavelength range, J. Synchrotron Radiat. 6, 64 (1999).
  52. Y. Tsusaka, K. Yokoyama, S. Takeda, M. Urakawa, Y. Kagoshima, J. Matsui, S. Kimura, H. Kimura, K. Kobayashi, and K. Izumi, Formation of parallel x-ray microbeam and its application, Jpn. J. Appl. Phys. 39, L635 (2000).
  53. K. Hirano and Y. Takahashi, Applications of x-ray magnifier and demagnifier to angle-resolved x-ray computed tomography, J. Phys.: Conf. Ser. 425, 192004 (2013).
  54. A. Authier, Dynamical Theory of X-ray Diffraction (Oxford University Press Inc., New York, 2004), Vol. 11, p. 189.
  55. E. Abbe, Beiträge zur Theorie des Mikroskops und der mikroskopischen Wahrnehmung, Archiv. Mikroskopische Anatomie 9, 413 (1873).
  56. M. Martinson, N. Samadi, B. Bassey, A. Gomez, and D. Chapman, Phase-preserving beam expander for biomedical x-ray imaging, J. Synchrotron Radiat. 22, 801 (2015).
  57. O. Márkus, I. Greving, E. Kornemann, M. Storm, F. Beckmann, J. Mohr, and A. Last, Optimizing illumination for full field imaging at high brilliance hard x-ray synchrotron sources, Opt. Express 26, 30435 (2018).
  58. C. Reinhard, M. Drakopoulos, S. I. Ahmed, H. Deyhle, A. James, C. M. Charlesworth, M. Burt, J. Sutter, S. Alexander, P. Garland et al., Beamline K11 DIAD: A new instrument for dual imaging and diffraction at diamond light source, J. Synchrotron Radiat. 28, 1985 (2021).
  59. T. Matsushita and H. Hashizume, Handbook on Synchrotron Radiation, edited by E. Koch (North Holland Publishing Company, Amsterdam, Netherlands, 1983), Vol. 1, Chap. 4.
  60. F. E. Christensen, A. Hornstrup, P. K. Frederiksen, S. Abdali, P. Grundsoe, H. W. Schnopper, R. A. Lewis, C. J. Hall, and K. N. Borozdin, Expanded beam x-ray optics calibration facility at the Daresbury Synchrotron, in Multilayer and Grazing Incidence X-ray/EUV Optics II (SPIE, Bellingham, Washington, 1994), Vol. 2011, pp. 540–548.
  61. C. Kamezawa, K. Hyodo, C. Tokunaga, T. Tsukada, and S. Matushita, Large-view x-ray imaging for medical applications using the world's only vertically polarized synchrotron radiation beam and a single asymmetric Si crystal, Phys. Med. Biol. 68, 195010 (2023).
  62. T. J. Davis, T. E. Gureyev, D. Gao, A. W. Stevenson, and S. W. Wilkins, X-ray image contrast from a simple phase object, Phys. Rev. Lett. 74, 3173 (1995).
  63. S. D. Shastri, Combining flat crystals, bent crystals and compound refractive lenses for high-energy x-ray optics, J. Synchrotron Radiat. 11, 150 (2004).
  64. A. Bravin, Exploiting the x-ray refraction contrast with an analyser: The state of the art, J. Phys. D 36, A24 (2003).
  65. F. Lenz and W. Scheffels, Das Zusammenwirken von Phasen- und Amplitudenkontrast in der elektronenmikroskopischen Abbildung, Z. Naturforsch. A 13, 226 (1958).
  66. E. J. Kirkland, Some image approximations, in Advanced Computing in Electron Microscopy (Springer, Cham, Switzerland, 2020), pp. 37–80.
  67. A. Momose and J. Fukuda, Phase-contrast radiographs of nonstained rat cerebellar specimen, Med. Phys. 22, 375 (1995).
  68. F. Thon, Zur Defokussierungsabhängigkeit des Phasenkontrastes bei der elektronenmikroskopischen Abbildung, Z. Naturforsch. A 21, 476 (1966).
  69. A. J. Morgan, K. T. Murray, M. Prasciolu, H. Fleckenstein, O. Yefanov, P. Villanueva-Perez, V. Mariani, M. Domaracky, M. Kuhn, S. Aplin, I. Mohacsi, M. Messerschmidt, K. Stachnik, Y. Du, A. Burkhart, A. Meents, E. Nazaretski, H. Yan, X. Huang, Y. S. Chu et al., Ptychographic x-ray speckle tracking with multi-layer Laue lens systems, J. Appl. Crystallogr. 53, 927 (2020).
  70. A. Rohou and N. Grigorieff, CTFFIND4: Fast and accurate defocus estimation from electron micrographs, J. Struct. Biol. 192, 216 (2015).
  71. R. Pretzsch, M. Dries, S. Hettler, M. Spiecker, M. Obermair, and D. Gerthsen, Investigation of hole-free phase plate performance in transmission electron microscopy under different operation conditions by experiments and simulations, Adv. Struct. Chem. Imaging 5, 5 (2019).
  72. X. Huang, A. Macrander, M. Honnicke, Y. Cai, and P. Fernandez, Dispersive spread of virtual sources by asymmetric x-ray monochromators, J. Appl. Crystallogr. 45, 255 (2012).
  73. R. Spiecker, Bragg magnifier optics for dose-efficient x-ray phase contrast imaging, Dissertation, Karlsruhe Institute of Technology, 2024, doi: 10.5445/IR/1000162889.
  74. Z. Huang and R. D. Ruth, Laser-electron storage ring, Phys. Rev. Lett. 80, 976 (1998).
  75. M. Bech, O. Bunk, C. David, R. Ruth, J. Rifkin, R. Loewen, R. Feidenhans’l, and F. Pfeiffer, Hard x-ray phase-contrast imaging with the compact light source based on inverse Compton x-rays, J. Synchrotron Radiat. 16, 43 (2009).
  76. B. Günther, R. Gradl, C. Jud, E. Eggl, J. Huang, S. Kulpe, K. Achterhold, B. Gleich, M. Dierolf, and F. Pfeiffer, The versatile x-ray beamline of the Munich Compact Light Source: Design, instrumentation and applications, J. Synchrotron Radiat. 27, 1395 (2020).
  77. B. Hornberger, J. Kasahara, R. Ruth, R. Loewen, and J. Khaydarov, Inverse Compton scattering x-ray source for research, industry and medical applications, in International Conference on X-ray Lasers 2020 (SPIE, Bellingham, Washington, 2021), Vol. 11886, pp. 51–60.
  78. C. G. Schroer, H.-C. Wille, O. H. Seeck, K. Bagschik, H. Schulte-Schrepping, M. Tischer, H. Graafsma, W. Laasch, K. Baev, S. Klumpp, R. Bartolini, H. Reichert, W. Leemans, and E. Weckert, The synchrotron radiation source PETRA III and its future ultra-low-emittance upgrade PETRA IV, Eur. Phys. J. Plus 137, 1312 (2022).
  79. P.-A. Douissard, A. Cecilia, X. Rochet, X. Chapel, T. Martin, T. van de Kamp, L. Helfen, T. Baumbach, L. Luquot, X. Xiao et al., A versatile indirect detector design for hard x-ray microimaging, J. Instrum. 7, P09016 (2012).
  80. M. Vogelgesang, T. Farago, T. F. Morgeneyer, L. Helfen, T. dos Santos Rolo, A. Myagotin, and T. Baumbach, Real-time image-content-based beamline control for smart 4D x-ray imaging, J. Synchrotron Radiat. 23, 1254 (2016).
  81. P. Modregger, D. Lübbert, P. Schäfer, R. Köhler, T. Weitkamp, M. Hanke, and T. Baumbach, Fresnel diffraction in the case of an inclined image plane, Opt. Express 16, 5141 (2008).
  82. S. Hrivňak, J. Uličný, and P. Vagovič, Fast fresnel propagation through a set of inclined reflecting planes applicable for x-ray imaging, Opt. Express 26, 34569 (2018).
  83. P. Vagovič, L. Švéda, A. Cecilia, E. Hamann, D. Pelliccia, E. Gimenez, D. Korytár, K. M. Pavlov, Z. Zápražný, M. Zuber et al., X-ray Bragg magnifier microscope as a linear shift invariant imaging system: Image formation and phase retrieval, Opt. Express 22, 21508 (2014).
  84. S. Hrivňak, J. Uličný, L. Mikeš, A. Cecilia, E. Hamann, T. Baumbach, L. Švéda, Z. Zápražný, D. Korytár, E. Gimenez-Navarro et al., Single-distance phase retrieval algorithm for Bragg magnifier microscope, Opt. Express 24, 27753 (2016).

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Phys. Rev. Research 7, 043315– Published 19 December, 2025
  • Received 28 October 2023
  • Revised 29 March 2024
  • Accepted 4 November 2025
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