Altermagnets exhibit characteristics akin to antiferromagnets, with spin-split anisotropic bands in momentum space. ${\mathrm{RuO}}_2$ has been considered as a prototype altermagnet; however, recent reports have questioned altermagnetic ground state in this material. In this Letter, we demonstrate spin-dependent tunneling magnetoresistance (TMR) in ${\mathrm{RuO}}_2$-based magnetic tunnel junctions, which suggests the spin-splitted anisotropic band structure of our ${\mathrm{RuO}}_2$ films. The observed TMR is contingent on the direction of the Néel vector of ${\mathrm{RuO}}_2$ and reverse its sign by the inversion of the Néel vector. These results reflect the altermagnetic nature of ${\mathrm{RuO}}_2$ and highlight its potential for spintronic applications, leveraging the combined strengths of ferromagnetic and antiferromagnetic systems.

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• Magnetic order
• Magnetotransport
• Spintronics
• Altermagnets
• X-ray diffraction