Effect of Gd3+ on the colloidal stability of liposomes

Juan Sabín, Gerardo Prieto, Simona Sennato, Juan M. Ruso, Roberta Angelini, Federico Bordi, and Félix Sarmiento
Phys. Rev. E 74, 031913 – Published 25 September 2006

Abstract

Lanthanide ions such as La3+ and Gd3+ are well known to have large effects on the structure of phospholipid membranes. Unilamellar vesicles of dipalmitoylphosphatidylcholine (DPPC) were prepared by sonication method and confirmed by transmission electron microscopy. The effects of concentration of gadolinium ions Gd3+ on DPPC unilamellar vesicles in aqueous media were studied by different techniques. As physical techniques, photon correlation spectroscopy, electrophoretic mobility, and differential scanning calorimetry were used. The theoretical predictions of the colloidal stability of liposomes were followed using the Derjaguin-Landau-Verwey-Overbeek theory. Changes in the size of liposomes and high polydispersities values were observed as Gd3+ concentration increases, suggesting that this cation induces the aggregation of vesicles. Electrophoretic mobility measurements on unilamellar vesicles as a function of Gd3+ ion concentration show that the vesicles adsorb Gd3+ ions. Above Gd3+ concentrations of 0.1moldm3, the ζ potential and light scattering measurements indicate the beginning of aggregation process. For comparison with similar phospholipids, the zeta potential of phosphatidylcholine interacting with Gd3+ was measured, showing an analogous behavior. Differential scanning calorimetry has been used to determine the effect of Gd3+ on the transition temperature (Tc) and on the enthalpy (ΔHc) associated with the process.

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  • Received 27 February 2006

DOI:https://doi.org/10.1103/PhysRevE.74.031913

©2006 American Physical Society

Authors & Affiliations

Juan Sabín1, Gerardo Prieto1,*, Simona Sennato2, Juan M. Ruso1, Roberta Angelini2, Federico Bordi2, and Félix Sarmiento1

  • 1Grupo de Biofísica e Interfases, Departamento de Física Aplicada, Facultade de Física, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain
  • 2Dipartimento di Fisica, Università di Roma “La Sapienza,” Piazzale Aldo Moro 2, I-00185 Roma, Italy and INFM CRS-SOFT, Unità di Roma1, Rome, Italy

  • *Corresponding author. FAX: +34 981 520 676. Electronic address: faxera@usc.es

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Vol. 74, Iss. 3 — September 2006

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Physical Review E Scope Description to Include Biological Physics
January 14, 2016

The editors of Physical Review E are pleased to announce that the journal’s stated scope has been expanded to explicitly include the term “Biological Physics.”

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